Your browser doesn't support javascript.
loading
Characterization of blood dendritic and regulatory T cells in asymptomatic adults with sub-microscopic Plasmodium falciparum or Plasmodium vivax infection.
Kho, Steven; Marfurt, Jutta; Handayuni, Irene; Pava, Zuleima; Noviyanti, Rintis; Kusuma, Andreas; Piera, Kim A; Burdam, Faustina H; Kenangalem, Enny; Lampah, Daniel A; Engwerda, Christian R; Poespoprodjo, Jeanne R; Price, Ric N; Anstey, Nicholas M; Minigo, Gabriela; Woodberry, Tonia.
Afiliação
  • Kho S; Global and Tropical Health Division, Menzies School of Health Research and Charles Darwin University, Darwin, Australia. steven.kho@menzies.edu.au.
  • Marfurt J; Global and Tropical Health Division, Menzies School of Health Research and Charles Darwin University, Darwin, Australia.
  • Handayuni I; Global and Tropical Health Division, Menzies School of Health Research and Charles Darwin University, Darwin, Australia.
  • Pava Z; Global and Tropical Health Division, Menzies School of Health Research and Charles Darwin University, Darwin, Australia.
  • Noviyanti R; Eijkman Institute for Molecular Biology, Jakarta, Indonesia.
  • Kusuma A; Eijkman Institute for Molecular Biology, Jakarta, Indonesia.
  • Piera KA; Global and Tropical Health Division, Menzies School of Health Research and Charles Darwin University, Darwin, Australia.
  • Burdam FH; Timika Malaria Research Programme, Papuan Health and Community Development Foundation, Timika, Papua, Indonesia.
  • Kenangalem E; Timika Malaria Research Programme, Papuan Health and Community Development Foundation, Timika, Papua, Indonesia.
  • Lampah DA; Rumah Sakit Umum Daerah Kabupaten Mimika, Timika, Papua, Indonesia.
  • Engwerda CR; Timika Malaria Research Programme, Papuan Health and Community Development Foundation, Timika, Papua, Indonesia.
  • Poespoprodjo JR; QIMR Berghofer Medical Research Institute, Brisbane, Australia.
  • Price RN; Timika Malaria Research Programme, Papuan Health and Community Development Foundation, Timika, Papua, Indonesia.
  • Anstey NM; Rumah Sakit Umum Daerah Kabupaten Mimika, Timika, Papua, Indonesia.
  • Minigo G; Department of Paediatrics, University of Gadjah Mada, Yogyakarta, Indonesia.
  • Woodberry T; Global and Tropical Health Division, Menzies School of Health Research and Charles Darwin University, Darwin, Australia.
Malar J ; 15: 328, 2016 06 21.
Article em En | MEDLINE | ID: mdl-27328659
BACKGROUND: Plasmodium falciparum and Plasmodium vivax infections compromise dendritic cell (DC) function and expand regulatory T (Treg) cells in both clinical disease (malaria) and experimental human sub-microscopic infection. Conversely, in asymptomatic microscopy-positive (patent) P. falciparum or P. vivax infection in endemic areas, blood DC increase or retain HLA-DR expression and Treg cells exhibit reduced activation, suggesting that DC and Treg cells contribute to the control of patent asymptomatic infection. The effect of sub-microscopic (sub-patent) asymptomatic Plasmodium infection on DC and Treg cells in malaria-endemic area residents remains unclear. METHODS: In a cross-sectional household survey conducted in Papua, Indonesia, 162 asymptomatic adults were prospectively evaluated for DC and Treg cells using field-based flow cytometry. Of these, 161 individuals (99 %) were assessed retrospectively by polymerase chain reaction (PCR), 19 of whom had sub-microscopic infection with P. falciparum and 15 with sub-microscopic P. vivax infection. Flow cytometric data were re-analysed after re-grouping asymptomatic individuals according to PCR results into negative controls, sub-microscopic and microscopic parasitaemia to examine DC and Treg cell phenotype in sub-microscopic infection. RESULTS: Asymptomatic adults with sub-microscopic P. falciparum or P. vivax infection had DC HLA-DR expression and Treg cell activation comparable to PCR-negative controls. Sub-microscopic P. falciparum infection was associated with lower peripheral CD4(+) T cells and lymphocytes, however sub-microscopic Plasmodium infection had no apparent effect on DC sub-set number or Treg cell frequency. CONCLUSIONS: In contrast to the impairment of DC maturation/function and the activation of Treg cells seen with sub-microscopic parasitaemia in primary experimental human Plasmodium infection, no phenotypic evidence of dysregulation of DC and Treg cells was observed in asymptomatic sub-microscopic Plasmodium infection in Indonesian adults. This is consistent with DC and Treg cells retaining their functional capacity in sub-microscopic asymptomatic infection with P. falciparum or P. vivax in malaria-endemic areas.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células Dendríticas / Malária Vivax / Malária Falciparum / Linfócitos T Reguladores / Infecções Assintomáticas Tipo de estudo: Observational_studies / Prevalence_studies / Risk_factors_studies Limite: Adult / Female / Humans / Male País como assunto: Asia Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células Dendríticas / Malária Vivax / Malária Falciparum / Linfócitos T Reguladores / Infecções Assintomáticas Tipo de estudo: Observational_studies / Prevalence_studies / Risk_factors_studies Limite: Adult / Female / Humans / Male País como assunto: Asia Idioma: En Ano de publicação: 2016 Tipo de documento: Article