Effect of breviscapine against hepatic ischemia reperfusion injury.
J Surg Res
; 203(2): 268-74, 2016 06 15.
Article
em En
| MEDLINE
| ID: mdl-27363631
ABSTRACT
BACKGROUND:
Breviscapine is an active ingredient extracted from traditional Chinese medicine Erigeron breviscapus. The purpose of this study was to investigate the effect of breviscapine injection on hepatic ischemia and/or reperfusion injury.METHODS:
Forty rats were randomly divided into five groups (n = 8) Sham group, Ischemia reperfusion 1 (I/R1) + normal saline (NS) group, I/R1 + breviscapine (Bre), I/R2 + NS group, and I/R2 + Bre group. Group1 and group2 represent ischemia time for 10 min and 30 min, respectively. Breviscapine or normal saline was administered to rats (single dose of 10 mg/Kg, intravenously) 30 min before hepatic ischemia. Serum transaminases, histopathologic changes, malondialdehyde (MDA), and superoxide dismutase (SOD) in liver tissues were evaluated. The expression level of mitochondrial fusion 2 (Mfn2) was also investigated.RESULTS:
After 24-h reperfusion, based on the histopathologic analysis, compared with NS control group, the liver function was improved in breviscapine group. Liver enzymes aspartate and alanine aminotransferase levels were significantly lower in the I/R + Bre group, when compared with the I/R + NS group. Pretreatment with breviscapine reduced MDA level (P < 0.05) and increased SOD activity significantly in I/R + Bre compared with I/R + NS group. Western blot and RT-q polymerase chain reaction showed that Mfn2 was significantly downregulated in breviscapine preconditioning group as compared to normal saline control group.CONCLUSIONS:
Breviscapine preconditioning attenuates liver ischemia reperfusion injury via inhibiting liver oxidative stress reaction. The protective mechanism probably inhibits Mfn2 protein and mRNA expression.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Flavonoides
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Medicamentos de Ervas Chinesas
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Traumatismo por Reperfusão
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Substâncias Protetoras
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Fígado
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article