Characterization of Viral Populations by Using Circular Sequencing.
J Virol
; 90(20): 8950-3, 2016 10 15.
Article
em En
| MEDLINE
| ID: mdl-27440878
ABSTRACT
With the enormous sizes viral populations reach, many variants are at too low a frequency to be detected by conventional next-generation sequencing (NGS) methods. Circular sequencing (CirSeq) is a method by which the error rate of next-generation sequencing is decreased so that even low-frequency viral variants can be accurately detected. The ability to visualize almost the entire genetic makeup of a viral swarm has implications for epidemiology, viral evolution, and vaccine design. Here we discuss experimental planning, analysis, and recent insights using CirSeq.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Virologia
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Vírus
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Metagenômica
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Sequenciamento de Nucleotídeos em Larga Escala
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article