Biosynthetic Gene Cluster for Surugamideâ
A Encompasses an Unrelated Decapeptide, Surugamideâ
F.
Chembiochem
; 17(18): 1709-12, 2016 09 15.
Article
em En
| MEDLINE
| ID: mdl-27443244
ABSTRACT
Genome mining is a powerful method for finding novel secondary metabolites. In our study on the biosynthetic gene cluster for the cyclic octapeptides surugamidesâ
A-E (inhibitors of cathepsinâ
B), we found a putative gene cluster consisting of four successive non-ribosomal peptide synthetase (NRPS) genes, surA, surB, surC, and surD. Prediction of amino acid sequence based on the NRPSs and gene inactivation revealed that surugamidesâ
A-E are produced by two NRPS genes, surA and surD, which were separated by two NRPS genes, surB and surC. The latter genes are responsible for the biosynthesis of an unrelated peptide, surugamideâ
F. The pattern of intercalation observed in the sur genes is unprecedented. The structure of surugamideâ
F, a linear decapeptide containing one 3-amino-2-methylpropionic acid (AMPA) residue, was determined by spectroscopic methods and was confirmed by solid-phase peptide synthesis.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos Cíclicos
/
Streptomyces
/
Família Multigênica
/
Genes Bacterianos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article