Your browser doesn't support javascript.
loading
Properties of skeletal muscle in the teleost Sternopygus macrurus are unaffected by short-term electrical inactivity.
Güth, Robert; Chaidez, Alexander; Samanta, Manoj P; Unguez, Graciela A.
Afiliação
  • Güth R; Department of Biology, New Mexico State University, Las Cruces, New Mexico; and.
  • Chaidez A; Department of Biology, New Mexico State University, Las Cruces, New Mexico; and.
  • Samanta MP; Systemix Institute, Redmond, Washington.
  • Unguez GA; Department of Biology, New Mexico State University, Las Cruces, New Mexico; and gunguez@nmsu.edu.
Physiol Genomics ; 48(9): 699-710, 2016 09 01.
Article em En | MEDLINE | ID: mdl-27449658
Skeletal muscle is distinguished from other tissues on the basis of its shape, biochemistry, and physiological function. Based on mammalian studies, fiber size, fiber types, and gene expression profiles are regulated, in part, by the electrical activity exerted by the nervous system. To address whether similar adaptations to changes in electrical activity in skeletal muscle occur in teleosts, we studied these phenotypic properties of ventral muscle in the electric fish Sternopygus macrurus following 2 and 5 days of electrical inactivation by spinal transection. Our data show that morphological and biochemical properties of skeletal muscle remained largely unchanged after these treatments. Specifically, the distribution of type I and type II muscle fibers and the cross-sectional areas of these fiber types observed in control fish remained unaltered after each spinal transection survival period. This response to electrical inactivation was generally reflected at the transcript level in real-time PCR and RNA-seq data by showing little effect on the transcript levels of genes associated with muscle fiber type differentiation and plasticity, the sarcomere complex, and pathways implicated in the regulation of muscle fiber size. Data from this first study characterizing the acute influence of neural activity on muscle mass and sarcomere gene expression in a teleost are discussed in the context of comparative studies in mammalian model systems and vertebrate species from different lineages.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fibras Musculares Esqueléticas Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fibras Musculares Esqueléticas Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article