Protein-peptide nutritional material prepared from surimi wash-water using immobilized chymotrypsin-trypsin.
J Sci Food Agric
; 97(6): 1746-1752, 2017 Apr.
Article
em En
| MEDLINE
| ID: mdl-27465270
ABSTRACT
BACKGROUND:
In the production process of surimi, large quantities of wastewater are produced. Thus it would be interesting to develop an efficient protocol for the recovery of protein from hairtail surimi wash-water.RESULTS:
A technique involving the use of immobilized chymotrypsin-trypsin (I-CT) was developed, providing a practical method for the preparation of protein-peptide nutritional material (PPNM). Under optimized reaction conditions, the recovery rate of nitrogen of surimi wash-water was measured as 98.3 ± 2.9%. Nutritional evaluation of the protein-peptide fraction demonstrated that it contained all essential amino acids (EAA) for humans, accounting for 44.1% of the total amino acid (TAA) content, which was determined to be 78.2 g per 100 g dry matter. The essential amino acid index (EAAI) and biological value (BV) were 101.7 (>95) and 76.7 respectively. A wide range of volatile flavor compounds (>50), including aldehydes, ketones, alcohols, hydrocarbons and heterocyclic compounds, were identified in PPNM by gas chromatography/mass spectrometry (GC/MS) analysis.CONCLUSION:
An efficient and practical protocol for the recovery of protein from hairtail surimi wash-water has been developed. The PPNM prepared in this work could be used as a nutraceutical and as an ingredient of functional foods. © 2016 Society of Chemical Industry.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Quimotripsina
/
Tripsina
/
Proteínas de Peixes
/
Águas Residuárias
/
Produtos Pesqueiros
Tipo de estudo:
Guideline
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article