Your browser doesn't support javascript.
loading
Oxidized Low-density Lipoprotein (ox-LDL) Cholesterol Induces the Expression of miRNA-223 and L-type Calcium Channel Protein in Atrial Fibrillation.
He, Fengping; Xu, Xin; Yuan, Shuguo; Tan, Liangqiu; Gao, Lingjun; Ma, Shaochun; Zhang, Shebin; Ma, Zhanzhong; Jiang, Wei; Liu, Fenglian; Chen, Baofeng; Zhang, Beibei; Pang, Jungang; Huang, Xiuyan; Weng, Jiaqiang.
Afiliação
  • He F; Department Institute of Cardiovascular Diseases, The Yuebei People's Hospital, Medical College, Shantou University, Shantou, Guangdong, China.
  • Xu X; Department Institute of Cardiovascular Diseases, The Yuebei People's Hospital, Medical College, Shantou University, Shantou, Guangdong, China.
  • Yuan S; Department Institute of Cardiovascular Diseases, The Yuebei People's Hospital, Medical College, Shantou University, Shantou, Guangdong, China.
  • Tan L; Department Institute of Cardiovascular Diseases, The Yuebei People's Hospital, Medical College, Shantou University, Shantou, Guangdong, China.
  • Gao L; Department Institute of Cardiovascular Diseases, The Yuebei People's Hospital, Medical College, Shantou University, Shantou, Guangdong, China.
  • Ma S; Department Institute of Cardiovascular Diseases, The Yuebei People's Hospital, Medical College, Shantou University, Shantou, Guangdong, China.
  • Zhang S; Department Institute of Cardiovascular Diseases, The Yuebei People's Hospital, Medical College, Shantou University, Shantou, Guangdong, China.
  • Ma Z; Department Institute of Cardiovascular Diseases, The Yuebei People's Hospital, Medical College, Shantou University, Shantou, Guangdong, China.
  • Jiang W; Department Institute of Cardiovascular Diseases, The Yuebei People's Hospital, Medical College, Shantou University, Shantou, Guangdong, China.
  • Liu F; Department Institute of Cardiovascular Diseases, The Yuebei People's Hospital, Medical College, Shantou University, Shantou, Guangdong, China.
  • Chen B; Department Institute of Cardiovascular Diseases, The Yuebei People's Hospital, Medical College, Shantou University, Shantou, Guangdong, China.
  • Zhang B; Department Institute of Cardiovascular Diseases, The Yuebei People's Hospital, Medical College, Shantou University, Shantou, Guangdong, China.
  • Pang J; Department Institute of Cardiovascular Diseases, The Yuebei People's Hospital, Medical College, Shantou University, Shantou, Guangdong, China.
  • Huang X; Department Institute of Cardiovascular Diseases, The Yuebei People's Hospital, Medical College, Shantou University, Shantou, Guangdong, China.
  • Weng J; Department Institute of Cardiovascular Diseases, The Yuebei People's Hospital, Medical College, Shantou University, Shantou, Guangdong, China.
Sci Rep ; 6: 30368, 2016 08 04.
Article em En | MEDLINE | ID: mdl-27488468
ABSTRACT
Atrial fibrillation (AF) is the most common sustained arrhythmia causing high morbidity and mortality. While changing of the cellular calcium homeostasis plays a critical role in AF, the L-type calcium channel α1c protein has suggested as an important regulator of reentrant spiral dynamics and is a major component of AF-related electrical remodeling. Our computational modeling predicted that miRNA-223 may regulate the CACNA1C gene which encodes the cardiac L-type calcium channel α1c subunit. We found that oxidized low-density lipoprotein (ox-LDL) cholesterol significantly up-regulates both the expression of miRNA-223 and L-type calcium channel protein. In contrast, knockdown of miRNA-223 reduced L-type calcium channel protein expression, while genetic knockdown of endogenous miRNA-223 dampened AF vulnerability. Transfection of miRNA-223 by adenovirus-mediated expression enhanced L-type calcium currents and promoted AF in mice while co-injection of a CACNA1C-specific miR-mimic counteracted the effect. Taken together, ox-LDL, as a known factor in AF-associated remodeling, positively regulates miRNA-223 transcription and L-type calcium channel protein expression. Our results implicate a new molecular mechanism for AF in which miRNA-223 can be used as an biomarker of AF rheumatic heart disease.
Assuntos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fibrilação Atrial / Canais de Cálcio Tipo L / MicroRNAs / Lipoproteínas LDL Tipo de estudo: Prognostic_studies Limite: Adult / Aged / Animals / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fibrilação Atrial / Canais de Cálcio Tipo L / MicroRNAs / Lipoproteínas LDL Tipo de estudo: Prognostic_studies Limite: Adult / Aged / Animals / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2016 Tipo de documento: Article