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Site-Specific Photolabeling of the IgG Fab Fragment Using a Small Protein G Derived Domain.
Kanje, Sara; von Witting, Emma; Chiang, Samuel C C; Bryceson, Yenan T; Hober, Sophia.
Afiliação
  • Kanje S; Department of Protein Technology, KTH - Royal Institute of Technology , SE-10691, Stockholm, Sweden.
  • von Witting E; Department of Protein Technology, KTH - Royal Institute of Technology , SE-10691, Stockholm, Sweden.
  • Chiang SC; HERM, Department of Medicine Huddinge, Karolinska Institute , SE-14157, Stockholm, Sweden.
  • Bryceson YT; HERM, Department of Medicine Huddinge, Karolinska Institute , SE-14157, Stockholm, Sweden.
  • Hober S; Department of Protein Technology, KTH - Royal Institute of Technology , SE-10691, Stockholm, Sweden.
Bioconjug Chem ; 27(9): 2095-102, 2016 09 21.
Article em En | MEDLINE | ID: mdl-27491005
Antibodies are widely used reagents for recognition in both clinic and research laboratories all over the world. For many applications, antibodies are labeled through conjugation to different reporter molecules or therapeutic agents. Traditionally, antibodies are covalently conjugated to reporter molecules via primary amines on lysines or thiols on cysteines. While efficient, such labeling is variable and nonstoichiometric and may affect an antibody's binding to its target. Moreover, an emerging field for therapeutics is antibody-drug conjugates, where a toxin or drug is conjugated to an antibody in order to increase or incorporate a therapeutic effect. It has been shown that homogeneity and controlled conjugation are crucial in these therapeutic applications. Here we present two novel protein domains developed from an IgG-binding domain of Streptococcal Protein G. These domains show obligate Fab binding and can be used for site-specific and covalent attachment exclusively to the constant part of the Fab fragment of an antibody. The two different domains can covalently label IgG of mouse and human descent. The labeled antibodies were shown to be functional in both an ELISA and in an NK-cell antibody-dependent cellular cytotoxicity assay. These engineered protein domains provide novel tools for controlled labeling of Fab fragments and full-length IgG.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Imunoglobulina G / Fragmentos Fab das Imunoglobulinas Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Imunoglobulina G / Fragmentos Fab das Imunoglobulinas Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article