Identification of distinct genes associated with seawater aspirationinduced acute lung injury by gene expression profile analysis.
Mol Med Rep
; 14(4): 3168-78, 2016 Oct.
Article
em En
| MEDLINE
| ID: mdl-27509884
ABSTRACT
Seawater aspirationinduced acute lung injury (ALI) is a syndrome associated with a high mortality rate, which is characterized by severe hypoxemia, pulmonary edema and inflammation. The present study is the first, to the best of our knowledge, to analyze gene expression profiles from a rat model of seawater aspirationinduced ALI. Adult male SpragueDawley rats were instilled with seawater (4 ml/kg) in the seawater aspirationinduced ALI group (S group) or with distilled water (4 ml/kg) in the distilled water negative control group (D group). In the blank control group (C group) the rats' tracheae were exposed without instillation. Subsequently, lung samples were examined by histopathology; total protein concentration was detected in bronchoalveolar lavage fluid (BALF); lung wet/dry weight ratios were determined; and transcript expression was detected by gene sequencing analysis. The results demonstrated that histopathological alterations, pulmonary edema and total protein concentrations in BALF were increased in the S group compared with in the D group. Analysis of differential gene expression identified up and downregulated genes in the S group compared with in the D and C groups. A gene ontology analysis of the differential gene expression revealed enrichment of genes in the functional pathways associated with neutrophil chemotaxis, immune and defense responses, and cytokine activity. Kyoto Encyclopedia of Genes and Genomes analysis revealed that the cytokinecytokine receptor interaction pathway was one of the most important pathways involved in seawater aspirationinduced ALI. In conclusion, activation of the cytokinecytokine receptor interaction pathway may have an essential role in the progression of seawater aspirationinduced ALI, and the downregulation of tumor necrosis factor superfamily member 10 may enhance inflammation. Furthermore, IL6 may be considered a biomarker in seawater aspirationinduced ALI.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Água do Mar
/
Lesão Pulmonar Aguda
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Transcriptoma
/
Pulmão
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article