An optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity.
Analyst
; 141(20): 5849-5856, 2016 Oct 21.
Article
em En
| MEDLINE
| ID: mdl-27517096
ABSTRACT
The polysialyltransferases are biologically important glycosyltransferase enzymes responsible for the biosynthesis of polysialic acid, a carbohydrate polymer that plays a critical role in the progression of several diseases, notably cancer. Having improved the chemical synthesis and purification of the fluorescently-labelled DMB-DP3 acceptor, we report optimisation and validation of a highly sensitive cell-free high-throughput HPLC-based assay for assessment of human polysialyltransferase activity.
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Base de dados:
MEDLINE
Assunto principal:
Sialiltransferases
/
Cromatografia Líquida de Alta Pressão
/
Ensaios de Triagem em Larga Escala
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article