Single-cell multimodal profiling reveals cellular epigenetic heterogeneity.
Nat Methods
; 13(10): 833-6, 2016 10.
Article
em En
| MEDLINE
| ID: mdl-27525975
ABSTRACT
Sample heterogeneity often masks DNA methylation signatures in subpopulations of cells. Here, we present a method to genotype single cells while simultaneously interrogating gene expression and DNA methylation at multiple loci. We used this targeted multimodal approach, implemented on an automated, high-throughput microfluidic platform, to assess primary lung adenocarcinomas and human fibroblasts undergoing reprogramming by profiling epigenetic variation among cell types identified through genotyping and transcriptional analysis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Análise de Sequência de DNA
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Heterogeneidade Genética
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Epigênese Genética
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Análise de Célula Única
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Sequenciamento de Nucleotídeos em Larga Escala
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article