[Action Mechanism of Chlorambucil against Mantle Cell Lymphoma Cell Line Jeko-1].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 24(4): 1066-70, 2016 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-27531776
ABSTRACT
OBJECTIVE:
To explore the action mechanism of chlorambucil against mantle cell lymphoma cell line Jeko-1.METHODS:
The effect of chlorambucil on Jeko-1 cell proliferation was measured by MTT method. The effect of chlorambucil on the apoptosis of Jeko-1 cell was detected by Hoechst staining and Annexin V-FITC dual staining. The activation of PI3K/AKT signaling pathway and the expression of BAX, BCL-2, procaspase 3, procaspase 8 and procaspase 9 were detected by Western blot.RESULTS:
0, 5, 10, 20 µmol/L chlorambucil could inhibit Jeko-1 cell proliferation at 24, 48, 72 h in a time- and dose-dependent manner. Chlorambucil of 0, 5, 10, 20 µmol/L increased the apoptotic rate of Jeko-1 cells, upregulated the expression of BAX, procaspase 3, procaspase 8, procaspase 9 and PI3K, increased the phosphorylation of AKT and down-regulated the expression of BCL-2.CONCLUSION:
The chlorambucil can induce the apoptosis of mantle cell lymphoma Jeko-1 cells via blocking PI3K/AKT signaling pathway.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Linfoma de Célula do Manto
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2016
Tipo de documento:
Article