Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors.
Angew Chem Int Ed Engl
; 55(40): 12440-4, 2016 09 26.
Article
em En
| MEDLINE
| ID: mdl-27554600
ABSTRACT
The ability to remotely trigger CRISPR/Cas9 activity would enable new strategies to study cellular events with greater precision and complexity. In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs). The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences and supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Guia de Cinetoplastídeos
/
Sistemas CRISPR-Cas
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article