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Inverse relationship between erythrocyte size and platelet reactivity in elderly.
Milovanovic, M; Nilsson, S; Winblad, B; Jelic, V; Behbahani, H; Shahnaz, T; Oweling, M; Järemo, P.
Afiliação
  • Milovanovic M; a Department of Internal Medicine, Vrinnevi Hospital , Norrköping , Sweden.
  • Nilsson S; b Department of Social and Welfare , Linköping University , Linköping , Sweden.
  • Winblad B; c Primary Health Care and Department of Medical and Health Sciences , Linköping University , Norrköping , Sweden.
  • Jelic V; d Department NVS, Center for Alzheimer Research, Division for Neurogeriatrics , Karolinska Institutet , Huddinge , Sweden.
  • Behbahani H; e Karolinska University Hospital, Geriatric Clinic, Clinical Trial Unit , Huddinge , Sweden.
  • Shahnaz T; d Department NVS, Center for Alzheimer Research, Division for Neurogeriatrics , Karolinska Institutet , Huddinge , Sweden.
  • Oweling M; e Karolinska University Hospital, Geriatric Clinic, Clinical Trial Unit , Huddinge , Sweden.
  • Järemo P; d Department NVS, Center for Alzheimer Research, Division for Neurogeriatrics , Karolinska Institutet , Huddinge , Sweden.
Platelets ; 28(2): 182-186, 2017 Mar.
Article em En | MEDLINE | ID: mdl-27557548
Previous work indicates that erythrocytes (RBCs) accumulate ß-amyloid X-40 (Aß40) in individuals with Alzheimer disease (AD) and to a lesser extent in healthy elderly. The toxin damages RBCs and increases their mean corpuscular volume (MCV). Furthermore, AD platelets demonstrate lower reactivity. This study investigated interactions between RBCs and platelets. Older individuals with moderate hypertension (n = 57) were classified into two groups, depending on MCV in whole blood: The MCVhigh group comprised individuals with higher MCV (n = 27; 97 ± 3(SD) fl) and MCVlow group had relatively lower MCV (n = 30; 90 ± 3(SD) fl). Flow cytometry was used to determine platelet reactivity, i.e., the surface binding of fibrinogen after provocation. Adenosine diphosphate (ADP) and a thrombin receptor-activating protein (TRAP-6) were used as agonists. Subsequently, blood cells were divided according to density into 17 subfractions. Intra-RBC Aß40 content was analyzed and in all platelet populations surface-bound fibrinogen was determined to estimate platelet in vivo activity. We found Aß40 inside RBCs of approximately 50% of participants, but the toxin did not affect MCV and platelet reactivity. In contrast, MCV associated inversely with platelet reactivity as judged from surface-attached fibrinogen after ADP (1.7 µmol/L) (p < 0.05) and TRAP-6 provocation (57 µmol/L (p = 0.01) and 74 µmol/L (p < 0.05)). In several density fractions (nos. 3, 4, 8, 11-13 (p < 0.05) and nos. 5-7 (p < 0.01)) MCV linked inversely with platelet-attached fibrinogen. In our community-dwelling sample, enhanced MCV associated with decreased platelet reactivity and lower in vivo platelet activity. It resembles RBCs and platelet behavior in AD-type dementia.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Plaquetas / Envelhecimento / Ativação Plaquetária / Eritrócitos Limite: Aged / Aged80 / Female / Humans / Male Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Plaquetas / Envelhecimento / Ativação Plaquetária / Eritrócitos Limite: Aged / Aged80 / Female / Humans / Male Idioma: En Ano de publicação: 2017 Tipo de documento: Article