DNA profile of dog feces as evidence to solve a homicide.
Leg Med (Tokyo)
; 22: 54-7, 2016 Sep.
Article
em En
| MEDLINE
| ID: mdl-27591540
ABSTRACT
Dog fecal samples were collected at the crime scene and from the shoes of the suspect to see whether they could be linked. DNA was genotyped using a 145bp fragment containing a 60bp hotspot region of the mitochondrial DNA (mtDNA) control region. Once the species origin was identified, sequences were aligned with the 23 canine haplotypes defined, showing that evidence and reference had 100% identity with haplotype 5. The frequency of haplotype 5 and the exclusion power of the reference population were 0.056 and 0.89, respectively. The forensic index showed that it was 20 times more likely that the evidence belonged to the reference dog than to some other unknown animal. The results support that the mtDNA hypervariable region 1 (HV1) is a good alternative for typing in trace or degraded casework samples when the STR panel fails, and demonstrate the utility of domestic animal samples to give additional information to solve human legal cases.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
DNA Mitocondrial
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Cães
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Fezes
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Homicídio
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Animais Domésticos
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article