CRISPR as a strong gene editing tool.
BMB Rep
; 50(1): 20-24, 2017 Jan.
Article
em En
| MEDLINE
| ID: mdl-27616359
ABSTRACT
Clustered regularly-interspaced short palindromic repeats (CRISPR) is a new and effective genetic editing tool. CRISPR was initially found in bacteria to protect it from virus invasions. In the first step, specific DNA strands of virus are identified by guide RNA that is composed of crRNA and tracrRNA. Then RNAse III is required for producing crRNA from pre-crRNA. In The second step, a crRNAtracrRNACas9 complex guides RNase III to cleave target DNA. After cleavage of DNA by CRISPR-Cas9, DNA can be fixed by Non- Homologous End Joining (NHEJ) and Homology Directed Repair (HDR). Whereas NHEJ is simple and random, HDR is much more complex and accurate. Gene editing by CRISPR is able to be applied to various biological field such as agriculture and treating genetic diseases in human. [BMB Reports 2017; 50(1) 20-24].
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Associadas a CRISPR
/
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
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Sistemas CRISPR-Cas
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Edição de Genes
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article