Identification of a gene involved in the regulation of hyphal growth of Epichloë festucae during symbiosis.
FEMS Microbiol Lett
; 363(19)2016 10.
Article
em En
| MEDLINE
| ID: mdl-27624305
ABSTRACT
Secreted proteins, those involved in cell wall biogenesis, are likely to play a role in communication in the symbiotic interaction between the fungal endophyte Epichloë festucae with perennial ryegrass (Lolium perenne), particularly given the close association between fungal hyphae and the plant cell wall. Our hypothesis was that secreted proteins are likely to be responsible for establishing and maintaining a normal symbiotic relationship. We analyzed an endophyte EST database for genes with predicted signal peptide sequences. Here, we report the identification and characterization of rhgA; a gene involved in the regulation of hyphal growth in planta In planta analysis of ΔrhgA mutants showed that disruption of rhgA resulted in extensive unregulated hyphal growth. This phenotype was fully complemented by insertion of the rhgA gene and suggests that rhgA is important for maintaining normal hyphal growth during symbiosis.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Simbiose
/
Proteínas Fúngicas
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Regulação Fúngica da Expressão Gênica
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Hifas
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Epichloe
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Endófitos
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article