A Protocol to Enhance INS1E and MIN6 Functionality-The Use of Theophylline.
Int J Mol Sci
; 17(9)2016 Sep 12.
Article
em En
| MEDLINE
| ID: mdl-27626415
ABSTRACT
In vitro research in the field of type I diabetes is frequently limited by the availability of a functional model for islets of Langerhans. This method shows that by the addition of theophylline to the glucose buffers, mouse insulinoma MIN6 and rat insulinoma INS1E pseudo-islets can serve as a model for islets of Langerhans for in vitro research. The effect of theophylline is dose- and cell line-dependent, resulting in a minimal stimulation index of five followed by a rapid return to baseline insulin secretion by reducing glucose concentrations after a first high glucose stimulation. This protocol solves issues concerning in vitro research for type I diabetes as donors and the availability of primary islets of Langerhans are limited. To avoid the limitations of using human donor material, cell lines represent a valid alternative. Many different ß cell lines have been reported, but the lack of reproducible responsiveness to glucose stimulation remains a challenge.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Teofilina
/
Ilhotas Pancreáticas
/
Diabetes Mellitus Tipo 1
/
Insulina
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article