Interaction of an esophageal MEG protein from schistosomes with a human S100 protein involved in inflammatory response.
Biochim Biophys Acta Gen Subj
; 1861(1 Pt A): 3490-3497, 2017 Jan.
Article
em En
| MEDLINE
| ID: mdl-27639541
ABSTRACT
BACKGROUND:
The Micro-Exon Gene-14 (MEG-14) displays a remarkable structure that allows the generation of antigenic variation in Schistosomes. Previous studies showed that the soluble portion of the MEG-14 protein displays features of an intrinsically disordered protein and is expressed exclusively in the parasite esophageal gland. These features indicated a potential for interaction with host proteins present in the plasma and cells from ingested blood.METHODS:
A yeast two-hybrid experiment using as bait the soluble domain of Schistosoma mansoni MEG-14 (sMEG-14) against a human leukocyte cDNA library was performed. Pull-down and surface plasmon resonance (SPR) experiments were used to validate the interaction between sMEG-14 and human S100A9. Synchrotron radiation circular dichroism (SRCD) were used to detect structural changes upon interaction between sMEG-14 and human S100A9. Feeding of live parasites with S100A9 attached to a fluorophore allowed the tracking of the fate of this protein in the parasite digestive system.RESULTS:
S100A9 interacted with sMEG-14 consistently in yeast two-hybrid assay, pull-down and SPR experiments. SRCD suggested that MEG-14 acquired a more regular structure as a result of the interaction with S100A9. Accumulation of recombinant S100A9 in the parasite's esophageal gland, when ingested by live worms suggests that such interaction may occur in vivo.CONCLUSION:
S100A9, a protein previously described to be involved in modulation of inflammatory response, was found to interact with sMEG-14. GENERALSIGNIFICANCE:
Our results allow proposing a mechanism involving MEG-14 for the parasite to block inflammatory signaling, which would occur upon release of S100A9 when ingested blood cells are lysed.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Schistosoma mansoni
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Proteínas S100
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Proteínas de Protozoários
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Esôfago
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Inflamação
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article