Alternative fluorescent labeling strategies for characterizing gram-positive pathogenic bacteria: Flow cytometry supported counting, sorting, and proteome analysis of Staphylococcus aureus retrieved from infected host cells.

Hildebrandt, Petra; Surmann, Kristin; Salazar, Manuela Gesell; Normann, Nicole; Völker, Uwe; Schmidt, Frank

Hildebrandt, Petra; Surmann, Kristin; Salazar, Manuela Gesell; Normann, Nicole; Völker, Uwe; Schmidt, Frank.

Afiliação

Hildebrandt P; Department Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
Surmann K; ZIK-FunGene University Medicine Greifswald, Department Functional Genomics, Greifswald, Germany.
Salazar MG; Department Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
Normann N; ZIK-FunGene University Medicine Greifswald, Department Functional Genomics, Greifswald, Germany.
Völker U; Department Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
Schmidt F; Department Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.

Cytometry A ; 89(10): 932-940, 2016 10.

Article em En | MEDLINE | ID: mdl-27643682

Assuntos

Corantes Fluorescentes/metabolismo; Interações Hospedeiro-Patógeno/fisiologia; Proteoma/metabolismo; Coloração e Rotulagem/métodos; Infecções Estafilocócicas/microbiologia; Staphylococcus aureus/metabolismo; Adulto; Idoso de 80 Anos ou mais; Proteínas de Bactérias/metabolismo; Linhagem Celular; DNA Bacteriano/genética; Células Epiteliais/metabolismo; Células Epiteliais/microbiologia; Feminino; Citometria de Fluxo/métodos; Humanos; Masculino; Proteômica/métodos; Infecções Estafilocócicas/metabolismo

Palavras-chave

S. aureus; cell counting and sorting; flow cytometry; fluorescence labeling of S. aureus; fluorescence microscopy; fluorescent vancomycin conjugate; gram-positive bacteria; internalization; proteomics

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Coloração e Rotulagem / Infecções Estafilocócicas / Staphylococcus aureus / Proteoma / Interações Hospedeiro-Patógeno / Corantes Fluorescentes Limite: Adult / Aged80 / Female / Humans / Male Idioma: En Ano de publicação: 2016 Tipo de documento: Article

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