Renoprotective effect of erythropoietin in zebrafish after administration of gentamicin: an immunohistochemical study for ß-catenin and c-kit expression.
J Nephrol
; 30(3): 385-391, 2017 Jun.
Article
em En
| MEDLINE
| ID: mdl-27679401
ABSTRACT
BACKGROUND:
Gentamicin is an aminoglycoside antibiotic widely used in the treatment of infections caused by Gram-negative bacteria. The main limitation to its therapeutic effectiveness is the potential nephrotoxicity. Erythropoietin has a tissue protective effect widely demonstrated in the kidney. The aim of the present study was to evaluate the renoprotective effects of erythropoietin in a model of zebrafish (Danio rerio) after administration of gentamicin.METHODS:
Sixty adult zebrafish were subdivided into three groups group A was treated with gentamicin; group B received gentamicin and, 24 h later, epoetin alpha; group C received drug diluent only. In order to analyze the renoprotective activity of erythropoietin, the expression of c-kit and ß-catenin was evaluated by immunohistochemistry.RESULTS:
Generally, the zebrafish renal tubule regenerates 15 days after an injury. Conversely, 7 days after gentamicin administration, animals treated with erythropoietin (group B) showed a better renal injury repair as documented by increased expression of ß-catenin, less degenerated tubules, greater number of centers of regeneration, positivity for c-kit only in immature-looking tubules and lymphohematopoietic cells.CONCLUSION:
The expression of c-kit and ß-catenin suggests that erythropoietin may exert a role in regeneration reducing the extent of tubular damage from the outset after gentamicin administration.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Regeneração
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Imuno-Histoquímica
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Gentamicinas
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Fator de Células-Tronco
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Substâncias Protetoras
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Proteínas de Peixe-Zebra
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Beta Catenina
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Epoetina alfa
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Nefropatias
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Túbulos Renais
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article