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An important role for adenine, cholera toxin, hydrocortisone and triiodothyronine in the proliferation, self-renewal and differentiation of limbal stem cells in vitro.
Yu, Min; Bojic, Sanja; Figueiredo, Gustavo S; Rooney, Paul; de Havilland, Julian; Dickinson, Anne; Figueiredo, Francisco C; Lako, Majlinda.
Afiliação
  • Yu M; Institute of Genetic Medicine, Newcastle University, UK.
  • Bojic S; Institute of Genetic Medicine, Newcastle University, UK.
  • Figueiredo GS; Institute of Genetic Medicine, Newcastle University, UK; Department of Ophthalmology, Royal Victoria Infirmary, Newcastle University, UK.
  • Rooney P; NHS Blood and Transplant, UK.
  • de Havilland J; Institute of Cellular Medicine, Newcastle University, UK.
  • Dickinson A; Institute of Cellular Medicine, Newcastle University, UK.
  • Figueiredo FC; Institute of Genetic Medicine, Newcastle University, UK; Department of Ophthalmology, Royal Victoria Infirmary, Newcastle University, UK.
  • Lako M; Institute of Genetic Medicine, Newcastle University, UK. Electronic address: Majlinda.Lako@ncl.ac.uk.
Exp Eye Res ; 152: 113-122, 2016 11.
Article em En | MEDLINE | ID: mdl-27693410
The cornea is a self-renewing tissue located at the front of the eye. Its transparency is essential for allowing light to focus onto the retina for visual perception. The continuous renewal of corneal epithelium is supported by limbal stem cells (LSCs) which are located in the border region between conjunctiva and cornea known as the limbus. Ex vivo expansion of LSCs has been successfully applied in the last two decades to treat patients with limbal stem cell deficiency (LSCD). Various methods have been used for their expansion, yet the most widely used culture media contains a number of ingredients derived from animal sources which may compromise the safety profile of human LSC transplantation. In this study we sought to understand the role of these components namely adenine, cholera toxin, hydrocortisone and triiodothyronine with the aim of re-defining a safe and GMP compatible minimal media for the ex vivo expansion of LSCs on human amniotic membrane. Our data suggest that all four components play a critical role in maintaining LSC proliferation and promoting LSC self-renewal. However removal of adenine and triiodothyronine had a more profound impact and led to LSC differentiation and loss of viability respectively, suggesting their essential role for ex vivo expansion of LSCs. Replacement of each of the components with GMP-grade reagents resulted in equal growth to non-GMP grade media, however an enhanced differentiation of LSCs was observed, suggesting that additional combinations of GMP grade reagents need to be tested to achieve similar or better level of LSC maintenance in the same manner as the traditional LSC media.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco / Tri-Iodotironina / Hidrocortisona / Adenina / Toxina da Cólera / Limbo da Córnea / Transplante de Células-Tronco Limite: Humans / Middle aged Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco / Tri-Iodotironina / Hidrocortisona / Adenina / Toxina da Cólera / Limbo da Córnea / Transplante de Células-Tronco Limite: Humans / Middle aged Idioma: En Ano de publicação: 2016 Tipo de documento: Article