Validation of three automated assays for total antioxidant capacity determination in canine serum samples.
J Vet Diagn Invest
; 28(6): 693-698, 2016 Nov.
Article
em En
| MEDLINE
| ID: mdl-27698166
ABSTRACT
We performed analytical validation of 3 automated assays of the total antioxidant capacity (TAC) in canine serum and evaluated their use in dogs with inflammatory bowel disease. The assays were based on the generation of a 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical cation (ABTSâ¢+) in aqueous media, which produces a blue-green color. The antioxidants present in the sample remove the chromogen in proportion to their concentrations. The assays differed mainly in the way in which this radical was produced. All 3 assays produced acceptable results in the analytical validation. However, only 2 of the assays were capable of detecting significantly different TAC values in healthy and diseased animals.
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Base de dados:
MEDLINE
Assunto principal:
Análise Química do Sangue
/
Doenças Inflamatórias Intestinais
/
Doenças do Cão
/
Antioxidantes
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article