Substrate profiling of Zika virus NS2B-NS3 protease.
FEBS Lett
; 590(20): 3459-3468, 2016 Oct.
Article
em En
| MEDLINE
| ID: mdl-27714789
ABSTRACT
Zika virus (ZIKV), isolated from macaques in Uganda in 1947, was not considered to be a dangerous human pathogen. However, this view has recently changed as ZIKV infections are now associated with serious pathological disorders including microcephaly and Guillain-Barré syndrome. Similar to other viruses in the Flaviviridae family, ZIKV expresses the serine protease NS3 which is responsible for viral protein processing and replication. Herein, we report the expression of an active NS3pro domain fused with the NS2B cofactor (NS2BLN NS3pro ) in a prokaryotic expression system and profile its specificity for synthesized FRET-type substrate libraries. Our findings pave way for screening potential intracellular substrates of NS3 and for developing specific inhibitors of this ZIKV protease.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Serina Endopeptidases
/
Proteínas não Estruturais Virais
/
Zika virus
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article