1H-NMR-based metabolomic study on toxicity of methomyl and methidathion in fish.
J Environ Sci Health B
; 51(12): 824-831, 2016 Dec.
Article
em En
| MEDLINE
| ID: mdl-27715651
ABSTRACT
A 1H-nuclear magnetic resonance (NMR) spectroscopy with multivariate analysis was applied to detect the toxicity of antiacetylcholinesterase insecticides, methomyl (methyl (1E)-N-(methylcarbamoyloxy)ethanimidothioate) and methidathion (3-(dimethoxyphosphinothioyl sulfanylmethyl)-5-methoxy-1,3,4-thiadiazol-2-one), using zebrafish (Danio rerio) and Chinese bleak (Aphyocypris chinensis). Generally, methomyl and methidathion have been believed not to highly accumulate in fish tissues. However, these pesticides showed their toxicity by altering patterns of whole-body metabolites in neurotransmitter balance, energy metabolism, oxidative stress, and muscle maintenance in low concentrations. We used Pearson correlation analysis to contextualize the metabolic markers in pesticide treated groups. We observed that the positive correlations of choline with acetate and betaine in untreated control were shifted to null correlations showing acetylcholinesterase specific toxicity. This research demonstrated the applicability and potential of NMR metabolomics in detecting toxic effects of insecticide with a modicum of concentrations in aquatic environment.
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Base de dados:
MEDLINE
Assunto principal:
Compostos Organotiofosforados
/
Peixe-Zebra
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Cyprinidae
/
Inseticidas
/
Metomil
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article