Designed miniaturization of microfluidic biosensor platforms using the stop-flow technique.
Analyst
; 141(21): 6073-6079, 2016 Oct 17.
Article
em En
| MEDLINE
| ID: mdl-27747319
ABSTRACT
Here, we present a novel approach to increase the degree of miniaturization as well as the sensitivity of biosensor platforms by the optimization of microfluidic stop-flow techniques independent of the applied detection technique (e.g. electrochemical or optical). The readout of the labeled bioassays, immobilized in a microfluidic channel, under stop-flow conditions leads to a rectangular shaped peak signal. Data evaluation using the peak height allows for a high level miniaturization of the channel geometries. To study the main advantages and limitations of this method by numerical simulations, a universally applicable model system is introduced for the first time. Consequently, proof-of-principle experiments were successfully performed with standard and miniaturized versions of an electrochemical biosensor platform utilizing a repressor protein-based assay for tetracycline antibiotics. Herein, the measured current peak heights are the same despite the sextuple reduction of the channel dimensions. Thus, this results in a 22-fold signal amplification compared to the constant flow measurements in the case of the miniaturized version.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Técnicas Analíticas Microfluídicas
/
Miniaturização
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article