Label-free LC-MS/MS shotgun proteomics to investigate the anti-inflammatory effect of rCC16.
Mol Med Rep
; 14(5): 4496-4504, 2016 Nov.
Article
em En
| MEDLINE
| ID: mdl-27748820
ABSTRACT
Clara cell protein (CC16) is an anti-inflammatory protein, which is expressed in the airway epithelium. It is involved in the development of airway inflammatory diseases, including chronic obstructive pulmonary disease and asthma. However, the exact molecular mechanism underlying its antiinflammatory action remains to be fully elucidated. The aim of the present study was to define the protein profiles of the antiinflammatory effect of CC16 in lipopolysaccharide (LPS)treated rat tracheal epithelial (RTE) cells using shotgun proteomics. Protein extracts were obtained from control RTE cells, RTE cells treated with LPS and RTE cells treated with LPS and recombinant CC16 (rCC16). Subsequent labelfree quantification and bioinformatics analyses identified 12 proteins that were differentially expressed in the three treatment groups as a cluster of five distinct groups according to their molecular functions. Five of the twelve proteins were revealed to be associated with the cytoskeleton Matrix metalloproteinase9, myosin heavy chain 10, actinrelated protein3 homolog, elongation factor 1α1 (EF1α1), and acidic ribosomal phosphoprotein P0. Five of the twelve proteins were associated with cellular proliferation DNAdependent protein kinase catalytic subunit, EF1α1, tyrosine 3monooxygenase, caspase recruitment domain (CARD) protein 12 and adenosylhomocysteinase (SAHH) 3. Three proteins were associated with gene regulation EF1α1, SAHH 3 and acidic ribosomal phosphoprotein P0. Three proteins were associated with inflammation Tyrosine 3monooxygenase, CARD protein 12 and statinrelated protein. ATPase (H+transporting, V1 subunit A, isoform 1) was revealed to be associated with energy metabolism, and uridine diphosphate glycosyltransferase 1 family polypeptide A8 with drug metabolism and detoxification. The identified proteins were further validated using reverse transcriptionquantitative polymerase chain reaction. These protein profiles, and their interacting protein network, may facilitate the elucidation of the molecular mechanisms underlying the antiinflammatory effects of CC16.
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Base de dados:
MEDLINE
Assunto principal:
Traqueia
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Uteroglobina
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Proteínas Recombinantes
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Células Epiteliais
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Inflamação
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article