High-Throughput Indirect Quantitation of 13C Enriched Metabolites Using 1H NMR.
Anal Chem
; 88(22): 11147-11153, 2016 11 15.
Article
em En
| MEDLINE
| ID: mdl-27749041
ABSTRACT
Nuclear magnetic resonance (NMR) spectroscopy is widely used in metabolomics to perform quantitative profiling of low-molecular weight compounds from biological specimens. The measurement of endogenous metabolites using NMR has proven to be a powerful tool to identify new metabolic biomarkers in physiological and pathological conditions, and to study and evaluate treatment efficiency. In this study we present a rapid approach to indirectly quantify 13C enriched molecules using one-dimensional (1D) 1H NMR. We demonstrate this approach using isotopically labeled [1,6-13C]glucose and in four different cell lines. We confirm the applicability of this approach for treatment follow-up, utilizing a renal cancer cell line with rapamycin as a tool compound to study changes in metabolic profiles. Finally, we validate the applicability of this method to study metabolic biomarkers from ex vivo tumor extracts, after infusion, using isotopically enriched glucose. Given the high throughput and increased sensitivity of direct-detect 1H NMR, this analytical approach provides an avenue for simple and rapid metabolic analysis of biological samples including blood, urine, and biopsies.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Metabolômica
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Ensaios de Triagem em Larga Escala
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Espectroscopia de Prótons por Ressonância Magnética
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article