Silica nanoparticles doped with anthraquinone for lung cancer phototherapy.
J Photochem Photobiol B
; 165: 1-9, 2016 Dec.
Article
em En
| MEDLINE
| ID: mdl-27755994
ABSTRACT
In the present study, SiO2 nanoparticles functionalized with 3-(2-aminoethylamino)propyl group (SiNP-AAP) were used, for the first time, to covalently bond rose bengal (SiNP-AAP-RB) or 9,10-anthraquinone-2-carboxylic acid (SiNP-AAP-OCAq). The functionalized SiNP were characterized by Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM); elemental analysis (CHN) for determination of the dye concentration; FTIR and UV-vis diffuse reflectance (DR-UV-vis) and a surface area study (BET). The functionalized SiNPs were applied in photodynamic therapy (PDT) against lung cancer cell lines. The evaluated cytotoxicity revealed 20-30% cell survival after 15min of PDT for both materials but the OCAq concentration was half of the RB nanomaterial. The phototoxicity was mainly related to oxidative stress generated in the cellular environment by singlet oxygen and by hydrogen abstraction as confirmed by the laser flash photolysis technique. The unprecedented results indicate that SiNP-AAP-OCAq is a possible system for promoting cell apoptosis by both type I and type II mechanisms.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Portadores de Fármacos
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Antraquinonas
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Fármacos Fotossensibilizantes
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Dióxido de Silício
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Nanopartículas
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Neoplasias Pulmonares
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article