A simple LC-MS method for the determination of iohexol and iothalamate in serum, using ioversol as an internal standard.
Clin Chim Acta
; 463: 96-102, 2016 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-27756544
ABSTRACT
BACKGROUND:
Chronic kidney disease (CKD) is diagnosed and explored through the determination of the glomerular filtration rate (GFR). Our goal was to develop a simple LC-MS method for the determination in serum of 2 popular GFR markers, contrast agents iohexol and iothalamate, for routine use and comparison studies between the two markers. A similar contrast agent, ioversol, was used as an internal standard and the method underwent a rigorous validation protocol based on ß-expectation tolerance intervals.METHODS:
We adapted the HPLC-UV method from Cavalier et al. to our LC-MS system. Data treatment for the validation was performed using Multiquant 3.0 (Sciex, Framingham, MA, USA) and e.noval 3.0 software (Arlenda, Liège, Belgium).RESULTS:
According to the validation results our method will give accurate and reliable results for concentrations ranging from 6.8 to 250µg/ml for iohexol and 6.15µg/ml to 250µg/ml for iothalamate. In our practice these intervals are sufficient to determine both compounds in most patient samples. Samples with higher detected concentrations can always be diluted into range.CONCLUSION:
With its internal standard and extensive validation, our method is now ready for routine and clinical research use.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ácidos Tri-Iodobenzoicos
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Iohexol
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Ácido Iotalâmico
Tipo de estudo:
Guideline
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article