[Glucagon-like peptide 1 improves learning and memory abilities of rats with type 2 diabetes].
Nan Fang Yi Ke Da Xue Xue Bao
; 36(10): 1345-1351, 2016 Oct 20.
Article
em Zh
| MEDLINE
| ID: mdl-27777196
ABSTRACT
OBJECTIVE:
To investigate the effect of glucagon-like peptide 1 (GLP-1) on cognitive dysfunction in diabetic rats.METHODS:
Male SD rats were randomly divided into normal control group, diabetes mellitus (DM) group, and GLP-1 treatment group. Rat models of type 2 diabetes were established by high-sugar and high-fat feeding and streptozotocin (STZ) injection, and 25 days after the onset of diabetes, GLP-1 was infused in GLP-1 treatment group at the rate of 30 pmol·kg-1·min-1 via a subcutaneous osmotic pump for 7 days. The learning and cognitive ability of the rats was assessed with Morris water maze test, and the expression of cognition-related genes in the hippocampus tissue was detected with real-time PCR, Western blotting and immunohistochemical staining.RESULTS:
Compared with the normal control group, the diabetic rats showed significantly decreased learning and memory abilities (P<0.05) with increased hippocampal expressions of APP, BACE1, Arc, ERK1/2, PKA, and PKC mRNAs (P<0.05) and Arc protein. Compared with diabetic rats, GLP-1-treated rats showed significantly improvements in the learning and memory function (P<0.05) with decreased expressions of APP, BACE1, Arc, ERK1/2, and PKA mRNAs (P<0.05) and Arc protein.CONCLUSION:
GLP-1 can improve cognitive dysfunctions in diabetic rats possibly by regulating the PKC, PKA, and ERK1/2 pathways and inhibiting Arc expression in the hippocampus.
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Base de dados:
MEDLINE
Assunto principal:
Diabetes Mellitus Experimental
/
Diabetes Mellitus Tipo 2
/
Peptídeo 1 Semelhante ao Glucagon
/
Aprendizagem
/
Memória
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2016
Tipo de documento:
Article