Description of Hydration Water in Protein (Green Fluorescent Protein) Solution.
J Am Chem Soc
; 139(3): 1098-1105, 2017 01 25.
Article
em En
| MEDLINE
| ID: mdl-27783480
ABSTRACT
The structurally and dynamically perturbed hydration shells that surround proteins and biomolecules have a substantial influence upon their function and stability. This makes the extent and degree of water perturbation of practical interest for general biological study and industrial formulation. We present an experimental description of the dynamical perturbation of hydration water around green fluorescent protein in solution. Less than two shells (â¼5.5 Å) were perturbed, with dynamics a factor of 2-10 times slower than bulk water, depending on their distance from the protein surface and the probe length of the measurement. This dependence on probe length demonstrates that hydration water undergoes subdiffusive motions (τ â q-2.5 for the first hydration shell, τ â q-2.3 for perturbed water in the second shell), an important difference with neat water, which demonstrates diffusive behavior (τ â q-2). These results help clarify the seemingly conflicting range of values reported for hydration water retardation as a logical consequence of the different length scales probed by the analytical techniques used.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Água
/
Proteínas de Fluorescência Verde
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article