Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins.
Nat Methods
; 13(12): 989-992, 2016 Dec.
Article
em En
| MEDLINE
| ID: mdl-27798609
ABSTRACT
We describe a red-shifted fluorescence resonance energy transfer (FRET) pair optimized for dual-color fluorescence lifetime imaging (FLIM). This pair utilizes a newly developed FRET donor, monomeric cyan-excitable red fluorescent protein (mCyRFP1), which has a large Stokes shift and a monoexponential fluorescence lifetime decay. When used together with EGFP-based biosensors, the new pair enables simultaneous imaging of the activities of two signaling molecules in single dendritic spines undergoing structural plasticity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Transferência Ressonante de Energia de Fluorescência
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Proteínas de Fluorescência Verde
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Imagem Óptica
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Corantes Fluorescentes
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Proteínas Luminescentes
Limite:
Animals
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Female
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Humans
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Pregnancy
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article