Phylogenetic analysis of full-length, early infection, hepatitis C virus genomes among people with intravenous drug use: the InC3 Study.
J Viral Hepat
; 24(1): 43-52, 2017 01.
Article
em En
| MEDLINE
| ID: mdl-27808453
ABSTRACT
Cross-continental phylogenetic analysis is important to understand subtle molecular differences of currently circulating hepatitis C virus (HCV) subtypes. Existence of such differences can be crucial in pursuing a universal hepatitis C vaccine. We characterized molecular epidemiology of early HCV infections identified across nine cohorts [North America (n=4), Australia (n=4) and Europe (n=1)] in the International Collaborative of Incident HIV and Hepatitis C in Injecting Cohorts (InC3 ). One hundred and ninety-two full-length HCV genomes were amplified from plasma of incident infections and subjected to next generation sequencing to establish the largest cross-continental, full-length acute HCV genomic data set available to date. Genomes from the most common subtypes (1a n=94, 2b n=15 and 3a n=68) were used in phylogenetic analysis. Using full genome trees, 78 sequences (44%) were found to lie within 29 phylogenetic clusters/pairs defined on the basis of molecular similarity of consensus sequences. Of these, 26 each had exclusively Australian or North American sequences indicating a strong geographical bias for molecular similarity. On further analysis of behavioural and demographic associations, binary logistic regression analysis showed that older age and non-Caucasian ethnicity were significantly associated with clustering. HCV probably evolves in micro-epidemics within geographically isolated communities.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Filogenia
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Abuso de Substâncias por Via Intravenosa
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Hepatite C
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Hepacivirus
Limite:
Adult
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Female
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Humans
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Male
País como assunto:
America do norte
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Europa
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Oceania
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article