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Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesity.
Song, Mun-Gyu; Lee, Hye-Jin; Jin, Bo-Yeong; Gutierrez-Aguilar, Ruth; Shin, Kyung-Ho; Choi, Sang-Hyun; Um, Sung Hee; Kim, Dong-Hoon.
Afiliação
  • Song MG; Department of Pharmacology, Korea University College of Medicine, Seoul, Republic of Korea.
  • Lee HJ; Department of Pharmacology, Korea University College of Medicine, Seoul, Republic of Korea.
  • Jin BY; Department of Pharmacology, Korea University College of Medicine, Seoul, Republic of Korea.
  • Gutierrez-Aguilar R; División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico; Laboratorio de Enfermedades Metabólicas: Obesidad y Diabetes, Hospital Infantil de México "Federico Gómez", Mexico City, Mexico.
  • Shin KH; Department of Pharmacology, Korea University College of Medicine, Seoul, Republic of Korea.
  • Choi SH; Department of Pharmacology, Korea University College of Medicine, Seoul, Republic of Korea.
  • Um SH; Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea. Electronic address: shum@skku.edu.
  • Kim DH; Department of Pharmacology, Korea University College of Medicine, Seoul, Republic of Korea. Electronic address: LDHKIM@korea.ac.kr.
Mol Metab ; 5(11): 1113-1120, 2016 11.
Article em En | MEDLINE | ID: mdl-27818937
OBJECTIVE: Adipose tissue (AT) expansion requires AT remodeling, which depends on AT angiogenesis. Modulation of AT angiogenesis could have therapeutic promise for the treatment of obesity. However, it is unclear how the capacity of angiogenesis in each adipose depot is affected by over-nutrition. Therefore, we investigated the angiogenic capacity (AC) of subcutaneous and visceral fats in lean and obese mice. METHODS: We compared the AC of epididymal fat (EF) and inguinal fat (IF) using an angiogenesis assay in diet-induced obese (DIO) mice and diet-resistant (DR) mice fed a high-fat diet (HFD). Furthermore, we compared the expression levels of genes related to angiogenesis, macrophage recruitment, and inflammation using RT-qPCR in the EF and IF of lean mice fed a low-fat diet (LFD), DIO mice, and DR mice fed a HFD. RESULTS: DIO mice showed a significant increase in the AC of EF only at 22 weeks of age compared to DR mice. The expression levels of genes related to angiogenesis, macrophage recruitment, and inflammation were significantly higher in the EF of DIO mice than in those of LFD mice and DR mice, while expression levels of genes related to macrophages and their recruitment were higher in the IF of DIO mice than in those of LFD and DR mice. Expression of genes related to angiogenesis (including Hif1a, Vegfa, Fgf1, Kdr, and Pecam1), macrophage recruitment, and inflammation (including Emr1, Ccr2, Itgax, Ccl2, Tnf, and Il1b) correlated more strongly with body weight in the EF of HFD-fed obese mice compared to that of IF. CONCLUSIONS: These results suggest depot-specific differences in AT angiogenesis and a potential role in the susceptibility to diet-induced obesity.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tecido Adiposo / Neovascularização Fisiológica / Obesidade Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tecido Adiposo / Neovascularização Fisiológica / Obesidade Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article