Archaeal orthologs of Cdc45 and GINS form a stable complex that stimulates the helicase activity of MCM.
Proc Natl Acad Sci U S A
; 113(47): 13390-13395, 2016 11 22.
Article
em En
| MEDLINE
| ID: mdl-27821767
ABSTRACT
The regulated recruitment of Cdc45 and GINS is key to activating the eukaryotic MCM(2-7) replicative helicase. We demonstrate that the homohexameric archaeal MCM helicase associates with orthologs of GINS and Cdc45 in vivo and in vitro. Association of these factors with MCM robustly stimulates the MCM helicase activity. In contrast to the situation in eukaryotes, archaeal Cdc45 and GINS form an extremely stable complex before binding MCM. Further, the archaeal GINSâ¢Cdc45 complex contains two copies of Cdc45. Our analyses give insight into the function and evolution of the conserved core of the archaeal/eukaryotic replisome.
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MEDLINE
Assunto principal:
Proteínas Cromossômicas não Histona
/
Archaea
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Proteínas de Ciclo Celular
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Proteínas de Manutenção de Minicromossomo
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article