Profiling the MicroRNA Payload of Exosomes Derived from Ex Vivo Primary Colorectal Fibroblasts.
Methods Mol Biol
; 1509: 115-122, 2017.
Article
em En
| MEDLINE
| ID: mdl-27826922
ABSTRACT
The tumor microenvironment is a heterogeneous and dynamic network that exists between cancer and stroma, playing a critical role in cancer progression. Certain tumorigenic signals such as microRNAs are derived from the stroma and conveyed to cancer cells (and vice versa) in nanoparticles called exosomes. Their identification and characterization is an important step in better understanding cellular cross talk and its consequences. To this end we describe how to culture primary ex vivo derived fibroblasts from colorectal tissue, isolate their exosomes, extract exosomal RNA and perform microRNA profiling.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Colorretais
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Perfilação da Expressão Gênica
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MicroRNAs
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Exossomos
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Fibroblastos
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article