Your browser doesn't support javascript.
loading
Recombinant human dihydroxyacetonephosphate acyl-transferase characterization as an integral monotopic membrane protein.
Piano, Valentina; Nenci, Simone; Magnani, Francesca; Aliverti, Alessandro; Mattevi, Andrea.
Afiliação
  • Piano V; Department of Biology and Biotechnology, University of Pavia, V. Ferrata 9, 27100, Pavia, Italy.
  • Nenci S; Department of Biology and Biotechnology, University of Pavia, V. Ferrata 9, 27100, Pavia, Italy.
  • Magnani F; Department of Biology and Biotechnology, University of Pavia, V. Ferrata 9, 27100, Pavia, Italy.
  • Aliverti A; Department of Biosciences, University of Milan, V. Celoria 26, 20133, Milan, Italy.
  • Mattevi A; Department of Biology and Biotechnology, University of Pavia, V. Ferrata 9, 27100, Pavia, Italy. Electronic address: andrea.mattevi@unipv.it.
Biochem Biophys Res Commun ; 481(1-2): 51-58, 2016 Dec 02.
Article em En | MEDLINE | ID: mdl-27836547
Although the precise functions of ether phospholipids are still poorly understood, significant alterations in their physiological levels are associated either to inherited disorders or to aggressive metastatic cancer. The essential precursor, alkyl-dihydroxyacetone phosphate (DHAP), for all ether phospholipids species is synthetized in two consecutive reactions performed by two enzymes sitting on the inner side of the peroxisomal membrane. Here, we report the characterization of the recombinant human DHAP acyl-transferase, which performs the first step in alkyl-DHAP synthesis. By exploring several expression systems and designing a number of constructs, we were able to purify the enzyme in its active form and we found that it is tightly bound to the membrane through the N-terminal residues.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pichia / Aciltransferases / Peroxissomos / Proteínas de Membrana Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pichia / Aciltransferases / Peroxissomos / Proteínas de Membrana Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article