Mouse Immunization with Purified Needle Proteins from Type III Secretion Systems and the Characterization of the Immune Response to These Proteins.
Methods Mol Biol
; 1531: 193-201, 2017.
Article
em En
| MEDLINE
| ID: mdl-27837493
ABSTRACT
Many Gram-negative pathogens utilize a type III secretion (T3S) system to directly deliver effector molecules into host eukaryotic cells to manipulate cellular processes. These surface-exposed syringe-like structures are highly conserved, necessary for pathogenesis, and hence are therapeutic targets against a number of Gram-negative pathogens. Here we describe a protocol for using purified needle proteins to immunize mice, and subsequently, ways to characterize the immune response to immunization.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Proteínas Recombinantes
/
Sistemas de Secreção Tipo III
Tipo de estudo:
Guideline
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article