Recent advances in high-throughput 13C-fluxomics.
Curr Opin Biotechnol
; 43: 104-109, 2017 02.
Article
em En
| MEDLINE
| ID: mdl-27838571
ABSTRACT
The rise of high throughput (HT) strain engineering tools accompanying the area of synthetic biology is supporting the generation of a large number of microbial cell factories. A current bottleneck in process development is our limited capacity to rapidly analyze the metabolic state of the engineered strains, and in particular their intracellular fluxes. HT 13C-fluxomics workflows have not yet become commonplace, despite the existence of several HT tools at each of the required stages. This includes cultivation and sampling systems, analytics for isotopic analysis, and software for data processing and flux calculation. Here, we review recent advances in the field and highlight bottlenecks that must be overcome to allow the emergence of true HT 13C-fluxomics workflows.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Metaboloma
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Biologia Sintética
/
Análise do Fluxo Metabólico
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article