Ruthenation of Non-stacked Guanines in DNA G-Quadruplex Structures: Enhancement of c-MYC Expression.
Angew Chem Int Ed Engl
; 55(50): 15615-15618, 2016 12 12.
Article
em En
| MEDLINE
| ID: mdl-27860057
ABSTRACT
Guanine quadruplexes (GQs) are compact four-stranded DNA structures that play a key role in the control of a variety of biological processes, including gene transcription. Bulky ruthenium complexes featuring a bipyridine, a terpyridine, and one exchangeable ligand ([Ru(terpy)(bpy)X]n+ ) are able to metalate exposed guanines present in the GQ of the c-MYC promoter region that are not involved in quadruplex base pairing. qRT-PCR and western-blot experiments indicated that the complexes promote a remarkable increase in the expression of this oncogene. We also show that exchangeable thioether ligands (X=RSR', Met) allow regulation of the metalating activity of the complex with visible light.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Rutênio
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Regulação para Cima
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Proteínas Proto-Oncogênicas c-myc
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Regiões Promotoras Genéticas
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Quadruplex G
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Complexos de Coordenação
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article