Characterization of a Cell-Penetrating Peptide with Potential Anticancer Activity.
ChemMedChem
; 12(1): 42-49, 2017 01 05.
Article
em En
| MEDLINE
| ID: mdl-27860402
ABSTRACT
Cell-penetrating peptides (CPPs) are still an interesting and viable alternative for drug delivery applications. CPPs contain considerably high amounts of positively charged amino acids, imparting them with cationic character. Tumor cells are characterized by an enhanced anionic nature of their membrane surface, a property that could be used by CPPs to target these cells. We recently identified a branched CPP that displays a high internalization capacity while exhibiting selectivity for certain tumor cell types. In this study we elucidated this observation in greater detail by investigating the underlying mechanism behind the cellular uptake of this peptide. An additional cytotoxicity screen against several cancer cell lines indeed demonstrates high cytotoxic activity against cancer cells over normal fibroblasts. Furthermore, we show that this feature can be used for delivering the anticancer drug actinomycinâ
D with high efficiency in the MCF-7 cancer cell line.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Dactinomicina
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Peptídeos Penetradores de Células
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Antineoplásicos
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article