Arp2/3 complex and Mps3 are required for regulation of ribosome biosynthesis in the secretory stress response.
Yeast
; 34(4): 155-163, 2017 04.
Article
em En
| MEDLINE
| ID: mdl-27862269
ABSTRACT
Secretory defects cause transcriptional repression of ribosome biogenesis in Saccharomyces cerevisiae. However, the molecular mechanism underlying secretory defect-induced transcriptional repression of ribosome biogenesis remains to be fully elucidated. In this study, we demonstrated that the Arp2/3 complex was required for reduction of ribosome protein gene expression in response to defective secretion by addition of tunicamycin. Two cmd1 mutants, cmd1-228 and cmd1-239 that cause mislocalization of calmodulin and defective mitotic spindle formation, respectively, failed to interact with Arc35, a component of the Arp2/3 complex. These mutants also caused defects in the reduction of ribosome protein gene expression induced by secretory blockade. A mutation in TUB4 (tub4-1), whose product has an essential function in microtubule organization, showed a similar response. In addition, we showed that the response to a secretory defect required SUN protein Mps3, which was localized at the nuclear envelope and involved in spindle pole body assembly. These results suggest that the Arp2/3 complex is required to transmit signals resulting from secretory blockade, and that the spindle pole body functions as a transit point from cytoplasm to Mps3 at the nuclear envelope. Copyright © 2016 John Wiley & Sons, Ltd.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ribossomos
/
Saccharomyces cerevisiae
/
Proteínas Nucleares
/
Regulação Fúngica da Expressão Gênica
/
Proteínas de Saccharomyces cerevisiae
/
Complexo 2-3 de Proteínas Relacionadas à Actina
/
Proteínas de Membrana
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article