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Epstein-Barr virus super-enhancer eRNAs are essential for MYC oncogene expression and lymphoblast proliferation.
Liang, Jun; Zhou, Hufeng; Gerdt, Catherine; Tan, Min; Colson, Tyler; Kaye, Kenneth M; Kieff, Elliott; Zhao, Bo.
Afiliação
  • Liang J; Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
  • Zhou H; Program in Virology, Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115.
  • Gerdt C; Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
  • Tan M; Program in Virology, Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115.
  • Colson T; Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
  • Kaye KM; Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
  • Kieff E; Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
  • Zhao B; Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
Proc Natl Acad Sci U S A ; 113(49): 14121-14126, 2016 12 06.
Article em En | MEDLINE | ID: mdl-27864512
ABSTRACT
Epstein-Barr virus (EBV) super-enhancers (ESEs) are essential for lymphoblastoid cell (LCL) growth and survival. Reanalyses of LCL global run-on sequencing (Gro-seq) data found abundant enhancer RNAs (eRNAs) being transcribed at ESEs. Inactivation of ESE components, EBV nuclear antigen 2 (EBNA2) and bromodomain-containing protein 4 (BRD4), significantly decreased eRNAs at ESEs -428 and -525 kb upstream of the MYC oncogene transcription start site (TSS). shRNA knockdown of the MYC -428 and -525 ESE eRNA caused LCL growth arrest and reduced cell growth. Furthermore, MYC ESE eRNA knockdown also significantly reduced MYC expression, ESE H3K27ac signals, and MYC ESEs looping to MYC TSS. These data indicate that ESE eRNAs strongly affect cell gene expression and enable LCL growth.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Proto-Oncogênicas c-myc / Elementos Facilitadores Genéticos / Herpesvirus Humano 4 / Interações Hospedeiro-Patógeno / Transtornos Linfoproliferativos Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Proto-Oncogênicas c-myc / Elementos Facilitadores Genéticos / Herpesvirus Humano 4 / Interações Hospedeiro-Patógeno / Transtornos Linfoproliferativos Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article