Overexpression of heat shock GroEL stress protein in leptospiral biofilm.
Microb Pathog
; 102: 8-11, 2017 Jan.
Article
em En
| MEDLINE
| ID: mdl-27865827
ABSTRACT
Leptospira is the causative agent of leptospirosis, which is an emerging zoonotic disease. Recent studies on Leptospira have demonstrated biofilm formation on abiotic surfaces. The protein expressed in the biofilm was investigated by using SDS-PAGE and immunoblotting in combination with MALDI-TOF mass spectrometry. The proteins expressed in Leptospira biofilm and planktonic cells was analyzed and compared. Among these proteins, one (60 kDa) was found to overexpress in biofilm as compared to the planktonic cells. MALDI-TOF analysis identified this protein as stress and heat shock chaperone GroEL. Our findings demonstrate that GroEL is associated with Leptospira biofilm. GroEL is conserved, highly immunogenic and a prominent stress response protein in pathogenic Leptospira spp., which may have clinical relevance.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
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Chaperonina 60
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Biofilmes
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Leptospira
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article