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A chemical proteomic probe for detecting native carrier protein motifs in nonribosomal peptide synthetases.
Kasai, Shota; Ishikawa, Fumihiro; Suzuki, Takehiro; Dohmae, Naoshi; Kakeya, Hideaki.
Afiliação
  • Kasai S; Department of System Chemotherapy and Molecular Sciences, Division of Bioinformatics and Chemical Genomics, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, Kyoto 606-8501, Japan. fishika@pharm.kyoto-u.ac.jp scseigyo-hisyo@pharm.kyoto-u.ac.jp.
  • Ishikawa F; Department of System Chemotherapy and Molecular Sciences, Division of Bioinformatics and Chemical Genomics, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, Kyoto 606-8501, Japan. fishika@pharm.kyoto-u.ac.jp scseigyo-hisyo@pharm.kyoto-u.ac.jp.
  • Suzuki T; Biomolecular Characterization Unit, RIKEN Center for Sustainable Resource Science, 2-1 Hirokawa, Wako, Saitama 351-0198, Japan.
  • Dohmae N; Biomolecular Characterization Unit, RIKEN Center for Sustainable Resource Science, 2-1 Hirokawa, Wako, Saitama 351-0198, Japan.
  • Kakeya H; Department of System Chemotherapy and Molecular Sciences, Division of Bioinformatics and Chemical Genomics, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, Kyoto 606-8501, Japan. fishika@pharm.kyoto-u.ac.jp scseigyo-hisyo@pharm.kyoto-u.ac.jp.
Chem Commun (Camb) ; 52(98): 14129-14132, 2016 Dec 01.
Article em En | MEDLINE | ID: mdl-27869277
Derivatization of a 5'-(vinylsulfonylaminodeoxy)adenosine scaffold with a clickable functionality provided an activity-based probe that was used to label native carrier protein (CP) motifs in nonribosomal peptide synthetases (NRPSs). When coupled with a fluorescent tag, this probe selectively targeted phosphopantetheinylated CPs (holo-form) from recombinant NRPS enzyme systems and in whole proteomes.
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Base de dados: MEDLINE Assunto principal: Peptídeo Sintases / Sondas Moleculares / Proteínas de Transporte / Adenosina / Proteômica Idioma: En Ano de publicação: 2016 Tipo de documento: Article
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PubMed Links

Base de dados: MEDLINE Assunto principal: Peptídeo Sintases / Sondas Moleculares / Proteínas de Transporte / Adenosina / Proteômica Idioma: En Ano de publicação: 2016 Tipo de documento: Article