A chemical proteomic probe for detecting native carrier protein motifs in nonribosomal peptide synthetases.
Chem Commun (Camb)
; 52(98): 14129-14132, 2016 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-27869277
Derivatization of a 5'-(vinylsulfonylaminodeoxy)adenosine scaffold with a clickable functionality provided an activity-based probe that was used to label native carrier protein (CP) motifs in nonribosomal peptide synthetases (NRPSs). When coupled with a fluorescent tag, this probe selectively targeted phosphopantetheinylated CPs (holo-form) from recombinant NRPS enzyme systems and in whole proteomes.
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Base de dados:
MEDLINE
Assunto principal:
Peptídeo Sintases
/
Sondas Moleculares
/
Proteínas de Transporte
/
Adenosina
/
Proteômica
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article