EGR2 is a gonadotropin-induced survival factor that controls the expression of IER3 in ovarian granulosa cells.
Biochem Biophys Res Commun
; 482(4): 877-882, 2017 Jan 22.
Article
em En
| MEDLINE
| ID: mdl-27890615
ABSTRACT
Pituitary gonadotropins are key hormones that orchestrate the growth and development of ovarian follicles. However, limited information is available on intra-ovarian factors that mediate the actions of gonadotropins. In this study, we identified that the early growth response 2 gene (EGR2) is a gonadotropin-inducible gene in granulosa cells of rats and humans. Analysis of consensus EGR-binding elements (EBEs) showed that the immediate early response 3 gene (IER3) is a novel transcriptional target gene of EGR2 as confirmed by the luciferase assay, electrophoretic mobility-shift assay (EMSA), chromatin immunoprecipitation (ChIP), and western blot analysis. Overexpression of EGR2 promoted survival of KGN human granulosa-derived cells in which IER3 acts as a mediator; knockdown of EGR2 induced death in KGN cells. Additionally, EGR2 was found to regulate the expression of myeloid cell leukemia 1 (MCL-1), which belongs to the BCL-2 family of proteins regulating cell survival. Thus, this study identified a novel signaling axis, comprised of gonadotropins-EGR2-IER3, which is important for the survival of granulosa cells during folliculogenesis.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ativação Transcricional
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Proteínas Reguladoras de Apoptose
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Proteína 2 de Resposta de Crescimento Precoce
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Gonadotropinas
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Células da Granulosa
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Proteínas de Membrana
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article