A Method for Determining the Content of Glycoproteins in Biological Samples.
Molecules
; 21(12)2016 Nov 26.
Article
em En
| MEDLINE
| ID: mdl-27898038
ABSTRACT
The glycoprotein purified from the mycelium extract of Tremella fuciformis was marked with iodine through the iodine substitution reaction. The content of iodine, which is indicative of the amount of the marked tremella glycoprotein (ITG), was detected with Inductively coupled plasma mass spectrometry (ICP-MS). The method was found to be stable, sensitive, and accurate at detecting the content of iodine-substituted glycoprotein, and was used in the quantitative analysis of biological samples, including blood and organs. Different biological samples were collected from rats after oral administration of ITG, and were tested for iodine content by ICP-MS to calculate the amount of ITG in the samples. The results suggested that ICP-MS is a sensitive, stable, and accurate method for detection of iodinated glycoproteins in blood and organs.
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Base de dados:
MEDLINE
Assunto principal:
Basidiomycota
/
Glicoproteínas
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article