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Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs.
Bertero, Alessandro; Pawlowski, Matthias; Ortmann, Daniel; Snijders, Kirsten; Yiangou, Loukia; Cardoso de Brito, Miguel; Brown, Stephanie; Bernard, William G; Cooper, James D; Giacomelli, Elisa; Gambardella, Laure; Hannan, Nicholas R F; Iyer, Dharini; Sampaziotis, Fotios; Serrano, Felipe; Zonneveld, Mariëlle C F; Sinha, Sanjay; Kotter, Mark; Vallier, Ludovic.
Afiliação
  • Bertero A; Wellcome Trust-MRC Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, CB2 0SZ, UK abertero@uw.edu lv225@cam.ac.uk.
  • Pawlowski M; Department of Surgery, University of Cambridge, Cambridge, CB2 0QQ, UK.
  • Ortmann D; Wellcome Trust-MRC Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, CB2 0SZ, UK.
  • Snijders K; Department of Clinical Neuroscience, University of Cambridge, Cambridge, CB2 0QQ, UK.
  • Yiangou L; Wellcome Trust-MRC Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, CB2 0SZ, UK.
  • Cardoso de Brito M; Department of Surgery, University of Cambridge, Cambridge, CB2 0QQ, UK.
  • Brown S; Wellcome Trust-MRC Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, CB2 0SZ, UK.
  • Bernard WG; Department of Surgery, University of Cambridge, Cambridge, CB2 0QQ, UK.
  • Cooper JD; Wellcome Trust-MRC Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, CB2 0SZ, UK.
  • Giacomelli E; Division of Cardiovascular Medicine, University of Cambridge, Cambridge, UK.
  • Gambardella L; Wellcome Trust-MRC Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, CB2 0SZ, UK.
  • Hannan NR; Department of Surgery, University of Cambridge, Cambridge, CB2 0QQ, UK.
  • Iyer D; Wellcome Trust-MRC Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, CB2 0SZ, UK.
  • Sampaziotis F; Department of Surgery, University of Cambridge, Cambridge, CB2 0QQ, UK.
  • Serrano F; Wellcome Trust-MRC Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, CB2 0SZ, UK.
  • Zonneveld MC; Division of Cardiovascular Medicine, University of Cambridge, Cambridge, UK.
  • Sinha S; Wellcome Trust-MRC Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, CB2 0SZ, UK.
  • Kotter M; Division of Cardiovascular Medicine, University of Cambridge, Cambridge, UK.
  • Vallier L; Wellcome Trust-MRC Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, CB2 0SZ, UK.
Development ; 143(23): 4405-4418, 2016 12 01.
Article em En | MEDLINE | ID: mdl-27899508
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Nucleares / Engenharia Genética / Proteínas com Domínio T / Fator 3 de Transcrição de Octâmero / Ciclina D / Proteínas Fetais / Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas / Sistemas CRISPR-Cas Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Nucleares / Engenharia Genética / Proteínas com Domínio T / Fator 3 de Transcrição de Octâmero / Ciclina D / Proteínas Fetais / Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas / Sistemas CRISPR-Cas Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article