Inflammatory protein response in CDKL5-Rett syndrome: evidence of a subclinical smouldering inflammation.
Inflamm Res
; 66(3): 269-280, 2017 Mar.
Article
em En
| MEDLINE
| ID: mdl-27900411
ABSTRACT
BACKGROUND:
Mutations in the cyclin-dependent kinase-like 5 gene cause a clinical variant of Rett syndrome (CDKL5-RTT). A role for the acute-phase response (APR) is emerging in typical RTT caused by methyl-CpG-binding protein 2 gene mutations (MECP2-RTT). No information is, to date, available on the inflammatory protein response in CDKL5-RTT. We evaluated, for the first time, the APR protein response in CDKL5-RTT.METHODS:
Protein patterns in albumin- and IgG-depleted plasma proteome from CDKL5-RTT patients were evaluated by two-dimensional gel electrophoresis/mass spectrometry. The resulting data were related to circulating cytokines and compared to healthy controls or MECP2-RTT patients. The effects of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) were evaluated.RESULTS:
CDKL5-RTT mutations resulted in a subclinical attenuated inflammation, specifically characterized by an overexpression of the complement component C3 and CD5 antigen-like, both strictly related to the inflammatory response. Cytokine dysregulation featuring a bulk increase of anti-inflammatory cytokines, predominantly IL-10, could explain the unchanged erythrocyte sedimentation rate and atypical features of inflammation in CDKL5-RTT. Omega-3 PUFAs were able to counterbalance the pro-inflammatory status.CONCLUSION:
For the first time, we revealed a subclinical smouldering inflammation pattern in CDKL5-RTT consisting in the coexistence of an atypical APR coupled with a dysregulated cytokine response.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Espasmos Infantis
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Síndrome de Rett
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Citocinas
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Reação de Fase Aguda
Limite:
Adolescent
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Child
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Child, preschool
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Female
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Humans
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Infant
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article